TSPSC Lab Technician Notification 2018 Syllabus

1. Units of measurement – different systems followed & their inter-conversions
2. Colorimetry – principles- complete understanding of percent transmittance, absorbance, & Beer Lambert’s law. Concept of using blank, standard and test (B, S& T). Mathematical calculation of quantity of an analyte through formulation of general formula.
3. Electrochemistry:- basic principles and applications of potentiometry. Ion selective electrodes.
4. Accidents and Emergencies & first-aid in the laboratory.
5. Bio-waste Management.
6. Automation :- basic principles, different components and general principles of usage applications
7. Ethical considerations:- transmission of ethical values, voluntariness, compliance.
8. Computers:- basic theoretical knowledge and usage of computers in clinical biochemistry laboratory.
9. Quality assurance :- Standard Operating Procedures (SOPs) – definition ,format,
10. Internal and external quality assessment –Introduction.

Unit-III: Biochemistry topics

Chemistry of carbohydrates and reactions of glucose. Carbohydrate metabolism, glycolysis, citric acid cycle, HMP shunt, oral glucose tolerance test in normal and diabetes mellitus, Ketone bodies. Chemistry of lipids and lipoproteins, Chemistry and metabolism of proteins –ammonia metabolism, urea cycle, Chemistryof nucleic acids- Structure of DNA,RNA, ATP, GTP, UTP., Non protein nitrogen substances (NPN) – urea, Creatinine, uric acid., Digestion and absorption of carbohydrates, lipids and proteins, Vitamins and minerals

Unit-IV: CLINICAL BIOCHEMISTRY

1. Acids, bases & salts:- Concepts of hydrogen ion concentration. pH & pH meter.
2. Plasma proteins – fractionation techniques, interpretation.
3. Bilirubin :- Outlines of formation and excretion. Conjugated and unconjugated bilirubin and its clinical applications. Jaundice.
4. Urine:- Formation and characteristics of normal urine. Abnormal constituents. Urine analysis and interpretation. Spot urinary protein, 24 hrs urinary protein.
5. Renal clearance tests ; & Total Protein & A:G ratio, Blood urea, S. Creatinie, BUN.
6. Body fluids:- Outlines of formation, composition and analysis of body fluids like cerebrospinal fluid, pleural fluid, & peritoneal fluid and interpretation of the results.
7. Glucose tolerance test :- Concepts of tolerance tests. Definition, patient preparation, performance, reporting and interpret ation of GTT.
8. Blood coagulation:- basic knowledge. Principles of estimation of plasma fibrinogen and prothrombin time, APTT.
9. Lipids & Lipoproteins:- triglycerides, cholesterol, phospholipids. Serum lipoprotein – patterns in health and disease.
10. Enzymes& Isoenzymes – LDH, CPK, GGT,SGOT, SGPT
11. Hormones – analysis of 17-keto steroids and VMA.
12. Inorganic ions:- Concepts; – Bicarbonates, phosphates, calcium, sodium, Potassium,
13. Blood gases Analysis:- Bicarbonate buffering system, Henderson-Hasselbach reaction, blood pH, pO2, pCO2, HCO3 ̄ calculations; nomograms

Unit-V: Demonstration topics

• a) liver function tests
• b) gastric analysis
• c) Renal function tests
• d) Radio-isotopic techniques
• e) Calculi formation and analysis
• f) Thyroid function tests
• g) Electrophoresis.

TSPSC Lab Technician II.CLINICAL MICROBIOLOGY Syllabus

Unit-I: General microbiology

Knowledge of various samples and procedures of collection for microscopy, serology & culture; Procedures of accepting/rejecting samples for cultures; Procedures of labeling, registering, transport & storage of various specimens; Procedures of laboratory work- safety and laboratory maintenance- Knowledge and expertise in working under conditions of biological hazard (BSL 1, 2, 3); Procedure of handling exposure to various infectious agents; Biomedical waste management; Maintenance of laboratory equipment; Culture methods for different bacteria & fungi – Media preparation; Methods of inoculation; Disposal of used media & specimens; Sterilization & Disinfection- Laboratory; Operation theatres.

Unit-II: Bacteriology

Characterization of aerobic and anaerobic bacteria – Differences between aerobic and anaerobic; Gram’s staining method-Reagents preparation; Alberts stainig-Reagents preparation; AFB staining-Reagents preparation; Characterization of Gram positive bacteria-cocci-Staphylococcus & streptococcus-bacilli-Mycobacteria, Clostridia & Corynebacterium; Gram negative bacteria-E.coli, Klebsiella, Proteus, Salmonella, Shigella, Vibrio, Pseudomonas; Principles of bacterial identification-staining, culture, spot tests, Commercial kits.

Unit.III: Mycology & Parasitology

Knowledge of common pathogenic fungi-differences between yeasts and moulds; Fungal media preparation, pH adjustment, preservation of media & samples, preparation of reagents-KOH, germ tube test, spot tests. Knowledge of various parasites and diseases caused by them; life cycles, morphology, hosts, vector, animal reservoir, epidemiology, parasite – host relationships, diagnosis and treatment – E.hystolytica, Giardia, Cryptosporidium, Ascaris, Echinococcosis, Schistosoma, Toxoplasma; Blood parasites – plasmodium, microfilaria; Light microscopy, reagent preparation, staining methods; Normal saline & Lugol iodine mounts ; Serological methods for parasite detection, identification of antigens in blood, feces and urine.

Unit.IV: Virology

Knowledge of medically important viruses; General laboratory tests for viral detection; Viral serology & ELISA; Tests based on Immunochrom atography; Maintenance of viral laboratory.

Unit.V: Automation in Microbial Identification

Automation in bacteriology-Blood culture systems; Methods of antimicrobial sensitivity tests- Disk Diffusion Test (Kirby Bauer) – MIC- E test; Principle of action of antibiotic agents; Detection of multi drug resistant (MDR) bacteria- ESBL, MRSA, VRE, Mycobacterium; Serological tests for antigen & antibody detection.

TSPSC Lab Technician III. PATHOLOGY Syllabus

Unit.I: CLINICAL PATHOLOGY

Urine collection, physical & chemical examination; Sugars, proteins, ketone bodies, bence jones proteins , bile, blood in urine; Micros copic examination – formed elements; Casts, crystals, parasites, abnormal cells; Other body fluids- types, names, physical & chemical examination; Semen analysis- physical, chemical and microscopic examination.

Unit.II: HEAMATOLOGY

Knowledge about RBCs, WBC, platelets and any abnormal cells; Haemoglobin estimation, red cell indices, haematocrit, ESR; Total cell counts- WBC, RBC, platelets, AEC; LE cell test Coagulation related tests; Haemoparasites, bone marrow slide preparation; Cell coulteroperation.

Unit.III: HISTOPATHOLOGY

Knowledge of material and equipments used; Collection, preservation and labeling of samples; Grossing methods, fixatives used and processing of tissue including bone tissue from collection to slide making; Special stains and immunohistochemistry; Staining procedures; Preservation of specimen, blocks, reports; Mounting of museum specimens.

Unit.IV: CYTOLOGY AND GENERAL PATHOLOGY

Different types of techniques and equipements used to obtain materials including various guided; methods- FNAC, imprint smear. Vaginal smear , buccal smear, cytospin cytosmears; Various staining methods, Different reagents used in Pathology; Preparation of fluids for cytological examination; Transport of specimen Preservation of samples., reports, records. Disposal of waste (infected materials.)

Unit.V: IMMUNO HAEMOTOLOGY

Material and methods used in Blood banking; Blood grouping techniques, ABO system and Rh system; Donors selection; Storage and processing of blood bags; Blood bags- grouping, quality control test and labeling; Direct and indirect coombs test, antibody titers; Problems in blood grouping and cross matching.

TSPSC Lab Technician Hall Ticket 2018

Hall Tickets can be downloaded 7 days before commencement of Examination.

TSPSC Lab Technician Exam Centre

1. The Examination will be held at HYDERABAD (including HMDA Jurisdiction) only or as may be decided by the Commission.
2. However, the Commission reserves the right to abolish / create new centre or centres for administrative reasons. Request for change of the centre will not be entertained.

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